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slug  (Proteintech)


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    Proteintech slug
    Slug, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/12129+1+ap/SNAI2+Antibody/pmc12924756-37-0-2
    Average 96 stars, based on 219 article reviews
    slug - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: LAPTM4B enhances the stemness of CD133 + liver cancer stem-like cells via WNT/β-catenin signaling
    Article Snippet: Antigens Manufacturer Application LAPTM4B ab242376, Abcam, USA 1:1000 for WB or 1:1000 for IHC or 1:50 for IF CD133 18470-1-AP, Proteintech Group, China 1:2000 for WB or 1:4000 for IHC or 1:50 for IF EpCAM #2929, Cell Signaling Technology, Beverly, MA 1:1000 for WB or 1:500 for IHC SOX9 #82630, Cell Signaling Technology, Beverly, MA 1:1000 for WB or 1:300 for IHC OCT4 #2750, Cell Signaling Technology, Beverly, MA 1:1000 for WB NANOG #4903, Cell Signaling Technology, Beverly, MA 1:1000 for WB β-catenin #8480, Cell Signaling Technology, Beverly, MA 1:1000 for WB or 1:150 for IHC or 1:100 for IF p-β-catenin #9565, Cell Signaling Technology, Beverly, MA 1:1000 for WB GSK-3β #12456, Cell Signaling Technology, Beverly, MA 1:1000 for WB p-GSK-3β #5558, Cell Signaling Technology, Beverly, MA 1:1000 for WB c-Myc #5605, Cell Signaling Technology, Beverly, MA 1:1000 for WB Cyclin D1 sc-20044, Santa Cruz Biotechnology 1:1000 for WB α-Tubulin 11224-1-AP, Proteintech Group, China 1:2000 for WB H3 17168-1-AP, Proteintech Group, China 1:2000 for WB Ubiquitin M026378, Abmart, China 1:1000 for WB E-cadherin 60335-1-lg, Proteintech Group, China 1:6000 for WB 27 Continued Antigens Manufacturer Application N-cadherin 66219-1-lg, Proteintech Group, China 1:10000 for WB vimentin 60330-1-lg, Proteintech Group, China 1:5000 for WB snail2 12129-1-AP, Proteintech Group, China 1:10000 for WB USP1 14346-1-AP, Proteintech Group, China 1:500 for WB or 2.5 ug for for IP USP14 14517-1-AP, Proteintech Group, China 1:2000 for WB or 2.5ug for IP DYKDDDDK tag Polyclonal antibody 20543-1-AP, Proteintech Group, China 1:20000 for WB or 2.5 ug for IP β-actin #4970, Cell Signaling Technology, Beverly, MA 1:1000 for WB Rat anti-Mouse IgG (H+L) Secondary Antibody, HRP 04-6020, Invitrogen, USA 1:1000 for WB HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) BA1055, BOSTER, China 1:5000 for WB Alexa 488- conjugated goat anti-mouse IgG ab150113, Abcam, USA 1:1000 for IF 28 Raw data for western blotting 29 30 31 32 33 34 35 36 37


    Article Title: LAPTM4B enhances the stemness of CD133 + liver cancer stem-like cells via WNT/β-catenin signaling
    Article Snippet: Antigens Manufacturer Application LAPTM4B ab242376, Abcam, USA 1:1000 for WB or 1:1000 for IHC or 1:50 for IF CD133 18470-1-AP, Proteintech Group, China 1:2000 for WB or 1:4000 for IHC or 1:50 for IF EpCAM #2929, Cell Signaling Technology, Beverly, MA 1:1000 for WB or 1:500 for IHC SOX9 #82630, Cell Signaling Technology, Beverly, MA 1:1000 for WB or 1:300 for IHC OCT4 #2750, Cell Signaling Technology, Beverly, MA 1:1000 for WB NANOG #4903, Cell Signaling Technology, Beverly, MA 1:1000 for WB β-catenin #8480, Cell Signaling Technology, Beverly, MA 1:1000 for WB or 1:150 for IHC or 1:100 for IF p-β-catenin #9565, Cell Signaling Technology, Beverly, MA 1:1000 for WB GSK-3β #12456, Cell Signaling Technology, Beverly, MA 1:1000 for WB p-GSK-3β #5558, Cell Signaling Technology, Beverly, MA 1:1000 for WB c-Myc #5605, Cell Signaling Technology, Beverly, MA 1:1000 for WB Cyclin D1 sc-20044, Santa Cruz Biotechnology 1:1000 for WB α-Tubulin 11224-1-AP, Proteintech Group, China 1:2000 for WB H3 17168-1-AP, Proteintech Group, China 1:2000 for WB Ubiquitin M026378, Abmart, China 1:1000 for WB E-cadherin 60335-1-lg, Proteintech Group, China 1:6000 for WB Continued Antigens Manufacturer Application N-cadherin 66219-1-lg, Proteintech Group, China 1:10000 for WB vimentin 60330-1-lg, Proteintech Group, China 1:5000 for WB snail2 12129-1-AP, Proteintech Group, China 1:10000 for WB USP1 14346-1-AP, Proteintech Group, China 1:500 for WB or 2.5 ug for for IP USP14 14517-1-AP, Proteintech Group, China 1:2000 for WB or 2.5ug for IP DYKDDDDK tag Polyclonal antibody 20543-1-AP, Proteintech Group, China 1:20000 for WB or 2.5 ug for IP β-actin #4970, Cell Signaling Technology, Beverly, MA 1:1000 for WB Rat anti-Mouse IgG (H+L) Secondary Antibody, HRP 04-6020, Invitrogen, USA 1:1000 for WB HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) BA1055, BOSTER, China 1:5000 for WB Alexa 488- conjugated goat anti-mouse IgG ab150113, Abcam, USA 1:1000 for IF Raw data for western blotting



    Article Title: Multi-Transcriptomic Analysis Reveals GSC-Driven MES-Like Differentiation via EMT in GBM Cell–Cell Communication
    Article Snippet: SNAI2/SLUG polyclonal antibody , Proteintech , 12129-1-AP.

    Incubation:

    Article Title: NOTCH2NLA silencing inhibits ovarian carcinoma progression and oncogenic activity in vivo and in vitro
    Article Snippet: Tissue sections were scanned using a Pannoramic MIDI digital slide scanner (3DHISTECH, Ltd., Hungary) to detect morphological differences in tumor cells, the area of tumor necrosis, and the number of necrotic foci. .. The tissue sections (5 μm) were deparaffinized, quenched with 3% hydrogen peroxide, antigen retrieved with EDTA, and incubated with 10% goat serum, then combined with the primary antibody 12129-1-AP (1:100), 20874-1-AP (1:100), 10366-1-AP (1:300), 12129-1-AP (1:300) (Proteintech, China) at 4 °C overnight. .. Sections were then incubated with secondary antibody ab205718 (1:2,000) (Abcam, UK) at 37 °C for 1 h, then visualized using EnVisionTM Detection Systems (Dako, Denmark).



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    Network pharmacology analysis of key interactions. (A) Venn diagram illustrating the shared targets of the Formononetin and TNBC. (B) The identification of key proteins was based on a PPI network. (C) Cluster analysis of the core gene <t>SNAI2.</t> (D) Construction of a network to illustrate the interactions between the Formononetin–TNBC targets. (E) GO and KEGG enrichment analysis.
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    Image Search Results


    Network pharmacology analysis of key interactions. (A) Venn diagram illustrating the shared targets of the Formononetin and TNBC. (B) The identification of key proteins was based on a PPI network. (C) Cluster analysis of the core gene SNAI2. (D) Construction of a network to illustrate the interactions between the Formononetin–TNBC targets. (E) GO and KEGG enrichment analysis.

    Journal: Food Science & Nutrition

    Article Title: Formononetin, a Key Component of Danggui Buxue Decoction, Inhibits Metastasis of Triple‐Negative Breast Cancer by Modulating SNAI2‐Driven EMT

    doi: 10.1002/fsn3.71286

    Figure Lengend Snippet: Network pharmacology analysis of key interactions. (A) Venn diagram illustrating the shared targets of the Formononetin and TNBC. (B) The identification of key proteins was based on a PPI network. (C) Cluster analysis of the core gene SNAI2. (D) Construction of a network to illustrate the interactions between the Formononetin–TNBC targets. (E) GO and KEGG enrichment analysis.

    Article Snippet: The membranes were incubated with primary antibodies SNAI2 (12129‐1‐AP, 1:5000, Proteintech), E‐Cadherin (20874‐1‐AP, 1:20000, Proteintech), α‐SMA (14395‐1‐AP, 1:1000, Proteintech), Collagen I (ab138492, 1:100, Abcam), Fibronectin (15613‐1‐AP, 1:200, Proteintech), and β‐actin ( AWA80001 , 1:500, Abiowell) overnight at 4°C.

    Techniques:

    Formononetin targeting SNAI2. (A) Molecular docking analysis was used to predict the binding of the active ingredient Formononetin to the core gene SNAI2. (B) The binding of Formononetin to SNAI2 was confirmed by the DARTS method. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. the Control group (0 μm/mL Formononetin + 0 μg/mL Pronase), # p < 0.05 vs. the Pronase group. Control group: Not treated with Pronase and Formononetin. Pronase group: Only treated with Pronase.

    Journal: Food Science & Nutrition

    Article Title: Formononetin, a Key Component of Danggui Buxue Decoction, Inhibits Metastasis of Triple‐Negative Breast Cancer by Modulating SNAI2‐Driven EMT

    doi: 10.1002/fsn3.71286

    Figure Lengend Snippet: Formononetin targeting SNAI2. (A) Molecular docking analysis was used to predict the binding of the active ingredient Formononetin to the core gene SNAI2. (B) The binding of Formononetin to SNAI2 was confirmed by the DARTS method. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. the Control group (0 μm/mL Formononetin + 0 μg/mL Pronase), # p < 0.05 vs. the Pronase group. Control group: Not treated with Pronase and Formononetin. Pronase group: Only treated with Pronase.

    Article Snippet: The membranes were incubated with primary antibodies SNAI2 (12129‐1‐AP, 1:5000, Proteintech), E‐Cadherin (20874‐1‐AP, 1:20000, Proteintech), α‐SMA (14395‐1‐AP, 1:1000, Proteintech), Collagen I (ab138492, 1:100, Abcam), Fibronectin (15613‐1‐AP, 1:200, Proteintech), and β‐actin ( AWA80001 , 1:500, Abiowell) overnight at 4°C.

    Techniques: Binding Assay, Control

    Formononetin inhibits cell proliferation, invasion, migration, and EMT by regulating SNAI2. (A) The effects of DBD and Formononetin on SNAI2 protein expression were detected by Western Blot. (B) The overexpression efficiency of oe‐SNAI2 was verified by qRT‐PCR. (C) The level of SNAI2 protein was detected by Western Blot. (D) Cell viability of TNBC cells was assessed by CCK‐8. (E) The migration of TNBC cells was assessed by the wound healing assay. (F) The invasion of TNBC cells was assessed by Transwell assay. (G) Expression of E‐cadherin, a‐SMA, Collagen I, and Fibronectin. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. the Control group. # p < 0.05 vs. the Formononetin + oe‐NC group. Control group: untreated TNBC cells. DBD group: TNBC cells were treated with DBD. Formononetin group: TNBC cells were treated with Formononetin. Formononetin + oe‐NC group: TNBC cells were treated with Formononetin and then transfected with oe‐NC. Formononetin + oe‐SNAI2 group: TNBC cells were treated with Formononetin and then transfected with oe‐SNAI2.

    Journal: Food Science & Nutrition

    Article Title: Formononetin, a Key Component of Danggui Buxue Decoction, Inhibits Metastasis of Triple‐Negative Breast Cancer by Modulating SNAI2‐Driven EMT

    doi: 10.1002/fsn3.71286

    Figure Lengend Snippet: Formononetin inhibits cell proliferation, invasion, migration, and EMT by regulating SNAI2. (A) The effects of DBD and Formononetin on SNAI2 protein expression were detected by Western Blot. (B) The overexpression efficiency of oe‐SNAI2 was verified by qRT‐PCR. (C) The level of SNAI2 protein was detected by Western Blot. (D) Cell viability of TNBC cells was assessed by CCK‐8. (E) The migration of TNBC cells was assessed by the wound healing assay. (F) The invasion of TNBC cells was assessed by Transwell assay. (G) Expression of E‐cadherin, a‐SMA, Collagen I, and Fibronectin. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. the Control group. # p < 0.05 vs. the Formononetin + oe‐NC group. Control group: untreated TNBC cells. DBD group: TNBC cells were treated with DBD. Formononetin group: TNBC cells were treated with Formononetin. Formononetin + oe‐NC group: TNBC cells were treated with Formononetin and then transfected with oe‐NC. Formononetin + oe‐SNAI2 group: TNBC cells were treated with Formononetin and then transfected with oe‐SNAI2.

    Article Snippet: The membranes were incubated with primary antibodies SNAI2 (12129‐1‐AP, 1:5000, Proteintech), E‐Cadherin (20874‐1‐AP, 1:20000, Proteintech), α‐SMA (14395‐1‐AP, 1:1000, Proteintech), Collagen I (ab138492, 1:100, Abcam), Fibronectin (15613‐1‐AP, 1:200, Proteintech), and β‐actin ( AWA80001 , 1:500, Abiowell) overnight at 4°C.

    Techniques: Migration, Expressing, Western Blot, Over Expression, Quantitative RT-PCR, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Control, Transfection